Composite

Part:BBa_K566039:Design

Designed by: José Eduardo Almeyda Carbajal, Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-28)


OL-pTetMnt-cI (Inverted)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 778
    Illegal BglII site found at 834
    Illegal BglII site found at 1008
    Illegal XhoI site found at 872
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 283
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Search for illegal sites was performed and it was optimized for E.coli codon usage


Source

Synthetic DNA

References